OPU-IVP技术在宁夏肉牛良种快速扩繁中的应用进展与展望

doi:10.19543/j.cnki.1673-4556.20260727.003

Abstract

Abstract:

As China's beef cattle genetic improvement work shifts from systematic infrastructure development to quality enhancement and efficiency breakthrough, the new National Beef Cattle Genetic Improvement Plan (2021–2035) has set higher requirements for core breeding population size, supply capacity of high-quality germplasm, and breeding efficiency. However, due to the constraints of the reproductive biological characteristics of cows, traditional methods such as artificial insemination, in vivo embryo production, and even natural mating are difficult to achieve rapid accumulation and efficient dissemination of high-quality genetic resources in a short period. At the present stage, the development and promotion of rapid breeding technologies for high-quality breeds is particularly important. Ovum pick-up and in vitro embryo production (OPU‑IVP) technology can significantly improve the oocyte utilization efficiency of elite cows, serving as a key technical support to break the "maternal propagation bottleneck" in beef cattle genetic improvement. In recent years, some local governments, university research institutions, and innovative enterprises in China have carried out practical applications of this technology in multiple regions, yet systematic summaries concerning regional models, application outcomes, and implementation bottlenecks remain limited. Based on a comprehensive analysis of the process of genetic improvement of beef cattle in China under the new circumstances, this paper systematically reviews the application progress of OPU‑IVP technology in the rapid propagation of elite beef cattle in Ningxia. By comparing typical domestic practice cases, including enterprise‑led, university‑enterprise collaborative, and industry‑university‑research platform models, we further summarize a regionally adaptable "Ningxia Model". Compared with the domestic enterprise-led, university‑enterprise collaborative, and industry-university-research platform models, the Ningxia model has relative advantages in technology implementation efficiency, resource integration, and regional adaptability. Current challenges in technology extension include insufficient technical standardization, cost control pressures, and poor integration with the industrial system. In the future, efforts should focus on optimizing culture systems, promoting domestic substitution of consumables, building regional service platforms, and improving benefit‑sharing and risk‑sharing mechanisms, so as to advance OPU‑IVP technology from "sporadic breakthroughs" to "full-scale rollout".

Key words: Ningxia beef cattle, Improved breed multiplication, OPU-IVP technology, Self-sufficiency in breeding stocks, Genetic improvement

CLC Number:

S823

Haisen Zhang, Haodong Xu, Fenli Zhang, Qi Gou, Jiahao Zhang, Ziang Wang, Junjun Deng, Yaping Jin, Jingning Li, Huatao Chen. Progress and prospects of OPU-IVP technology in the rapid propagation of high-quality beef cattle breeds in Ningxia[J].Chinese Livestock and Poultry Breeding, 2026, 22(10): 15-27.

Figures/Tables 4

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Table 1

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References 55

[1]	全国肉牛遗传改良计划（2021—2035年）[J]. 畜牧产业, 2021(7): 16-19.
	National beef cattle genetic improvement plan (2021-2035) [J]. Animal Husbandry Industry, 2021(7): 16-19.
[2]	周菊. 肉牛遗传改良关键技术的应用与效益分析[J]. 畜牧业环境, 2025(7): 5-6.
	ZHOU J. Application and benefit analysis of key technologies for beef cattle genetic improvement[J]. Animal Industry and Environment, 2025(7): 5-6.
[3]	刘玲茹. 肉牛遗传改良关键技术的推广与应用[J]. 畜牧业环境, 2024(22): 10-11.
	LIU L R. Promotion and application of key technologies for beef cattle genetic improvement[J]. Animal Industry and Environment, 2024(22): 10-11.
[4]	罗与同. 安格斯牛育种现状及优化策略研究[J]. 特种经济动植物, 2024, 27(10): 89-91.
	LUO Y T. Research on breeding status and optimization strategy of Angus cattle[J]. Special Economic Animal and Plant, 2024, 27(10): 89-91.
[5]	王延斌. 奶牛肉牛育种创新要由“点”到“面”[N]. 科技日报, 2021-08-11(006).
	WANG Y B. Dairy and beef cattle breeding innovation should move from "point" to "surface"[N]. Science and Technology Daily, 2021-08-11(006).
[6]	何小平. 肉牛遗传改良技术与繁殖效率的提升策略[J]. 畜牧业环境, 2025(14): 7-8.
	HE X P. Beef cattle genetic improvement technology and reproductive efficiency promotion strategy[J]. Animal Industry and Environment, 2025(14): 7-8.
[7]	邱金龙, 史延, 李爽, 等. 牛活体采卵和体外胚胎生产技术的应用现状和展望[J]. 浙江大学学报(农业与生命科学版), 2022, 48(5): 557-565, 604.
	QIU J L, SHI Y, LI S, et al. Application status and prospect of bovine ovum pick-up and in vitro embryo production technologies[J]. Journal of Zhejiang University (Agriculture & Life Sciences), 2022, 48(5): 557-565, 604.
[8]	杨成文, 张元来, 张杰. 肉牛体外胚胎移植中OPU-IVF技术的实践与效果分析[J]. 甘肃畜牧兽医, 2024, 54(6): 10-13.
	YANG C W, ZHANG Y L, ZHANG J. Practice and effect analysis of OPU-IVF technology in in vitro embryo transfer of beef cattle[J]. Gansu Animal Husbandry and Veterinary Medicine, 2024, 54(6): 10-13.
[9]	姚顺, 赵明礼, 张明, 等. 牛活体采卵技术研究进展[J]. 中国奶牛, 2020(1): 19-23.
	YAO S, ZHAO M L, ZHANG M, et al. Research progress of ovum pick-up on cattle[J]. China Dairy Cattle, 2020(1): 19-23.
[10]	鄂志强, 金敬栋, 高青山. OPU技术应用对牛产业的推动作用[J]. 农业技术与装备, 2020(8): 149-150.
	E Z Q, JIN J D, GAO Q S. The role of OPU technology application in promoting cattle industry[J]. Agricultural Technology & Equipment, 2020(8): 149-150.
[11]	刘燕飞. 高效繁育技术在牛羊品种改良中的应用[J]. 畜牧业环境, 2024(23): 5-6.
	LIU Y F. Application of efficient breeding technology in cattle and sheep variety improvement[J]. Animal Industry and Environment, 2024(23): 5-6.
[12]	任菊秋, 牛聪, 郭志福, 等. 牛羊优良品种繁育的遗传选择方法比较研究[J]. 畜牧业环境, 2024(6): 11-13.
	REN J Q, NIU C, GUO Z F, et al. A comparative study on genetic selection methods for breeding excellent breeds of cattle and sheep[J]. Animal Industry and Environment, 2024(6): 11-13.
[13]	王秀琴, 岳彩娟, 马小明, 等. 宁夏肉牛产业发展现状及高质量发展的思考[J]. 当代畜牧, 2021(10): 81-84.
	WANG X Q, YUE C J, MA X M, et al. Thoughts on the present situation and high-quality development of beef cattle industry in Ningxia[J]. Contemporary Animal Husbandry, 2021(10): 81-84.
[14]	刘春. 2025年全区畜牧业生产形势稳中向好[EB/OL]. 宁夏回族自治区农业农村厅, 2026-01-30 [2026‑05‑05].
	LIU C. Stable and improving production situation of animal husbandry in the region in 2025[EB/OL]. Department of Agriculture and Rural Affairs of Ningxia Hui Autonomous Region, 2026-01-30 [2026‑05‑05].
[15]	艾琦, 张坤, 蒋秋斐, 等. 宁夏肉牛产业高质量发展对策研究[J]. 畜牧业环境, 2024(10): 147-149.
	AI Q, ZHANG K, JIANG Q F, et al. Research on countermeasures for high quality development of beef cattle industry in Ningxia[J]. Animal Industry and Environment, 2024(10): 147-149.
[16]	张坤. 我区种公牛培育工作再上新台阶[EB/OL]. 宁夏回族自治区农业农村厅, 2025-11-12 [2026-05-05].
	ZHANG K. Our district's breeding bull cultivation work reaches a new level[EB/OL]. Department of Agriculture and Rural Affairs of Ningxia Hui Autonomous Region, 2025-11-12 [2026-05-05].
[17]	顾亚荣. “宁夏肉牛种群高效利用及优异性状选育关键技术研究”项目顺利通过验收[EB/OL]. 宁夏回族自治区农业农村厅, 2025-11-28 [2026-05-05].
	GU Y R. The project "Research on key technologies for efficient utilization and excellent trait breeding of Ningxia beef cattle population" successfully passed the acceptance[N/OL]. Department of Agriculture and Rural Affairs of Ningxia Hui Autonomous Region, 2025-11-28 [2026-05-05].
[18]	马云, 谷帅锋, 潘翠丽, 等. 宁夏回族自治区肉牛种业现状、问题及对策建议[J]. 中国畜禽种业, 2023, 19(2): 9-13.
	MA Y, GU S F, PAN C L, et al. Present situation, problems and countermeasures of beef cattle seed industry in Ningxia Hui Autonomous Region[J]. The Chinese Livestock and Poultry Breeding, 2023, 19(2): 9-13.
[19]	SALEK F, GUEST A, JOHNSON C, et al. Factors affecting the success of ovum pick-up, in vitro production and cryopreservation of embryos in cattle[J]. Animals, 2025, 15(3): 344.
[20]	SAKAGUCHI K. Optimization of ovum pick-up-in vitro fertilization and in vitro growth of immature oocytes in ruminants[J]. The Journal of Reproduction and Development, 2025, 71(1): 1-9.
[21]	MULYATI S, MUSTOFA I, SRIANTO P, et al. Insulin-like growth factor 1 and estrogen from cumulus cell culture increases the success of in vitro fertilization in cattle[J]. Open Veterinary Journal, 2025, 15(7): 3024-3034.
[22]	AMARAL H B S, SILVEIRA M M, NICOLÁS A C C V, et al. Melatonin improves bovine embryo production and quality via antioxidant, metabolic, and epigenetic pathways[J]. Antioxidants, 2025, 14(11): 1322.
[23]	YILDIZ M, CETIN Y. The effect of L-carnitine supplementation in culture medium on the development and cryopreservation of in vitro produced bovine embryos[J]. Veterinary Medicine and Science, 2025, 11(3): e70376.
[24]	LI Y F, WEI Y C, YANG M M, et al. Metabolomics of follicular fluid reveals the impact of heat stress on oocyte pick-up and in vitro embryo production efficiency in dairy cows[J]. Journal of Animal Science, 2025, 103: skaf216.
[25]	PYTEL A T, ŻYŻYŃSKA-GALEŃSKA K, GAJEWSKI Z, et al. Factors defining developmental competence of bovine oocytes collected for in vitro embryo production[J]. Biology of Reproduction, 2024, 111(1): 1-10.
[26]	ANCHORDOQUY J P, BALBI M, FARNETANO N A, et al. Effect of follicular wave synchronization using a progesterone-releasing intravaginal device-based protocol on in vitro embryo production in Bos taurus cows subjected to 14-day intervals ovum pick-up[J]. Animal Reproduction Science, 2024, 261: 107397.
[27]	MOTTA J C L, HAYDEN C B, SALA R V, et al. Advances in synchronization and superstimulation for OPU/IVEP: optimizing oocyte quantity and quality[J]. Reproduction, Fertility and Development, 2024, 37: RD24143.
[28]	李赫强. 牛体外生产关键技术的研究[D]. 杨凌: 西北农林科技大学, 2021.
	LI H Q. Research on key technologies of bovine in vitro production[D]. Yangling: Northwest A&F University, 2021.
[29]	黄聪. 奶牛OPU卵巢刺激程序的优化与应用[D]. 杨凌: 西北农林科技大学, 2024.
	HUANG C. Optimization and application of ovarian stimulation protocols for bovine OPU[D]. Yangling: Northwest A&F University, 2024.
[30]	李紫元. 富血小板血浆对奶牛超数排卵的作用和机制研究[D]. 杨凌: 西北农林科技大学, 2024.
	LI Z Y. Effects and mechanisms of platelet-rich plasma on superovulation in dairy cows[D]. Yangling: Northwest A&F University, 2024.
[31]	金梦冬. 奶牛OPU-IVP生产效率影响因素分析及卵巢刺激对卵泡微环境与早期胚胎发育的影响[D]. 杨凌: 西北农林科技大学, 2025.
	JIN M D. Analysis of factors affecting production efficiency of OPU-IVP in dairy cows and effects of ovarian stimulation on follicular microenvironment and early embryonic development[D]. Yangling: Northwest A&F University, 2025.
[32]	段春, 王强, 李萌. 中卫市海原县强化良种繁育做优肉牛产业“芯片”[EB/OL]. 科创中国, 2025-04-24 [2026-05-05].
	DUAN C, WANG Q, LI M. Haiyuan County of Zhongwei City strengthens improved breed multiplication and optimizes the "chip" of beef cattle industry[EB/OL]. Kechuang China, 2025-04-24 [2026-05-05].
[33]	赵建新, 苏政, 陆宗伟, 等. 胚胎移植引种在规模化肉牛养殖场的研究和示范[J]. 中国畜禽种业, 2023, 19(1): 76-79.
	ZHAO J X, SU Z, LU Z W, et al. Research and demonstration of embryo transfer introduction in large-scale beef cattle farms[J]. The Chinese Livestock and Poultry Breeding, 2023, 19(1): 76-79.
[34]	谢成运. 重组牛生长分化因子9蛋白对牛活体采卵控制性卵巢刺激的效果及其机制探究[D]. 杨凌: 西北农林科技大学, 2025.
	XIE C Y. Effects of recombinant bovine growth differentiation factor 9 protein on controlled ovarian stimulation for ovum pick-up in cattle and its mechanism investigation[D]. Yangling: Northwest A&F University, 2025.
[35]	欧四海, 杨阳, 王洪涛, 等. 乳肉牛融合发展创新模式与实践[J]. 中国奶牛, 2024(7): 72-77.
	OU S H, YANG Y, WANG H T, et al. Innovative model and practice of integrated development of dairy and beef cattle[J]. China Dairy Cattle, 2024(7): 72-77.
[36]	杨松, 李慧, 孙芳, 等. 荷斯坦牛及其与安格斯牛杂交后代屠宰性能和肉品质的比较[J]. 中国畜牧兽医, 2025, 52(3): 1211-1221.
	YANG S, LI H, SUN F, et al. Comparison of slaughter performance and meat quality between Holstein cattle and their crossbred offspring with Angus cattle[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1211-1221.
[37]	张爽, 梁怡, 段忠意, 等. 德系西门塔尔牛F1代的生产性能初步调查[J]. 北京农学院学报, 2021, 36(3): 41-49.
	ZHANG S, LIANG Y, DUAN Z Y, et al. Preliminary investigation on production performance of F1 generation of German Simmental cattle[J]. Journal of Beijing University of Agriculture, 2021, 36(3): 41-49.
[38]	GOTOH T, TAKAHASHI H, NISHIMURA T, et al. Meat produced by Japanese Black cattle and Wagyu[J]. Animal Frontiers, 2014, 4(4): 46-54.
[39]	张焓. 奶牛性控胚胎体外生产技术的优化[D]. 杨凌: 西北农林科技大学, 2024.
	ZHANG H. Optimization of in vitro production technology for sexed embryos in dairy cows[D]. Yangling: Northwest A&F University, 2024.
[40]	郝凌峰. 从胚胎到餐桌，看青岛肉牛产业探寻“出圈”路[N]. 农民日报, 2025-03-18.
	HAO L F. From embryo to table: exploring the "breakthrough" path of Qingdao beef cattle industry[N]. Farmers' Daily, 2025-03-18.
[41]	耿婷婷. 攻关胚胎技术，青岛企业实现黑毛和牛“借腹生子”[N]. 青岛日报, 2024-03-29.
	GENG T T. Overcoming embryo technology challenges: Qingdao enterprise achieves surrogate pregnancy for Japanese Black cattle[N]. Qingdao Daily, 2024-03-29.
[42]	孙桂东. 青岛和牛火出圈！一头能卖 20万，最多可年产3万头[N]. 半岛都市报, 2025-04-08.
	SUN G D. Qingdao Wagyu goes viral! Each sells for 200,000 yuan, annual output up to 30,000 heads[N]. Peninsula Metropolis Daily, 2025-04-08.
[43]	徐光勇, 刘振田, 陈祥飞, 等. 和牛OPU卵母细胞体外受精后胚胎发育潜能研究[J]. 中国畜牧兽医, 2025, 52(2): 730-739.
	XU G Y, LIU Z T, CHEN X F, et al. Study on embryonic developmental potential of embryos derived from ovum pickup oocytes and fertilization in vitro in wagyu[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 730-739.
[44]	徐鑫, 冯丽. 西北农林科大与地方、企业联合开展奶牛育种技术攻关:把课堂设在牛舍把人才留在行业[N]. 中国教育报, 2024-05-15.
	XU X, FENG L. Northwest A&F University collaborates with local government and enterprises on dairy cattle breeding technology research: setting classrooms in barns, retaining talents in the industry[N]. China Education Daily, 2024-05-15.
[45]	银川市科学技术协会. 科协助力·科创筑梦｜把实验室搬进牛舍——灵武奶牛科技小院破解精准育种难题[EB/OL]. 银川科普网, 2026-02-11 [2026-05-05].
	YINCHUAN ASSOCIATION FOR SCIENCE AND TECHNOLOGY. Science association empowers, technology creates dreams: moving laboratories into barns - Lingwu dairy cattle science and technology backyard solves precision breeding challenges [EB/OL]. Yinchuan Science Popularization Network, 2026-02-11 [2026-05-05].
[46]	中国科协. 连获两个奖项！科技小院成技术转化“关键枢纽”[EB/OL]. 科普中国, 2025-10-31 [2026-05-05].
	CHINA ASSOCIATION FOR SCIENCE AND TECHNOLOGY. Winning two awards! Science and technology backyard becomes "key hub" for technology transformation [EB/OL]. Science Popularization China, 2025-10-31 [2026-05-05].
[47]	山东省国资委. 奥克斯“试管奶牛”产业化取得重大突破[EB/OL]. 山东省人民政府国有资产监督管理委员会, 2019-04-24 [2026-05-05].
	SHANDONG STATE-OWNED ASSETS SUPERVISION AND ADMINISTRATION COMMISSION. Aoxing achieves major breakthrough in industrialization of "test-tube dairy cattle"[EB/OL]. Shandong SASAC, 2019-04-24 [2026-05-05].
[48]	新牧网. 山东省产学研联合攻关推动“试管奶牛”核心技术产业化[EB/OL]. 新牧网, 2019-04-27 [2026-05-05].
	XINMU NETWORK. Shandong's industry-university-research joint攻关 promotes industrialization of core "test-tube dairy cattle" technologies[EB/OL]. Xinmu Network, 2019-04-27 [2026-05-05].
[49]	我国奶牛繁育技术取得重大突破[J]. 乳业科学与技术, 2019, 42(2): 封三.
	Major breakthrough in dairy cattle breeding technology in China[J]. Journal of Dairy Science and Technology, 2019, 42(2): Inside Back Cover.
[50]	王方. “试管奶牛”破品种改良“缺芯之痛”[N]. 中国科学报, 2019-05-15.
	WANG F. "Test-tube dairy cattle" breaks the "core shortage pain" of breed improvement[N]. China Science Daily, 2019-05-15.
[51]	闪电新闻. 奶牛体外胚胎产业化技术成果发布会暨核心种质自主创新研讨会在济南召开[EB/OL]. 闪电新闻, 2019-04-22 [2026-05-05].
	SHANDIAN NEWS. Dairy cattle in vitro embryo industrialization technology achievement release conference and core germplasm independent innovation seminar held in Jinan[EB/OL]. Shandian News, 2019-04-22 [2026-05-05].
[52]	昝林森, 田成山, 王洪宝. 中国肉牛种业现状、存在问题及发展建议[J]. 家畜生态学报, 2012, 33(2): 4-7.
	ZAN L S, TIAN C S, WANG H B. Status quo and development of beef cattle breeding industry in China[J]. Journal of Domestic Animal Ecology, 2012, 33(2): 4-7.
[53]	栾曼茹, 张志浩, 闵佳, 等. 牛活体采卵及体外胚胎生产技术研究进展[J]. 中国牛业科学, 2025, 51(5): 63-68.
	LUAN M R, ZHANG Z H, MIN J, et al. Research progress on technology of ovum pick up and in vitro embryo production in cattle[J]. China Cattle Science, 2025, 51(5): 63-68.
[54]	DU Y, XIA Y, XU J, et al. Effects of donor age and reproductive history on developmental potential of ovum pickup oocytes in Japanese Black cattle (Wagyu)[J]. Theriogenology, 2024, 221: 25-30.
[55]	HANSEN P J. Review: some challenges and unrealized opportunities toward widespread use of the in vitro-produced embryo in cattle production[J]. animal, 2023, 17: 100745.

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技术手段 Technical means	单头母牛年后代数offspring per year/头	扩繁速度 Propagation speed	技术成本 Cost	应用阶段 The current application phase
自然交配Natural mating	≈1	慢	低	普遍
人工授精Artificial insemination	≈1	慢	低	普遍
超数排卵胚胎移植Superovulation and embryo transfer	5～10	中	中	部分应用
活体采卵胚胎移植OPU-IVP-ET	20～50	快	中高	快速发展

Progress and prospects of OPU-IVP technology in the rapid propagation of high-quality beef cattle breeds in Ningxia

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[11]	Zixin Jiang, Jiashu Tao, Zhisheng Hu, Enliang Song, Yuhan Liu, Liping Hu. The current situation and prospects of genetic improvement of beef cattle in Shandong province [J]. Chinese Livestock and Poultry Breeding, 2026, 22(10): 55-63.
[12]	Xue Bai, Fen Li, Yun Ma. The 14th Five-Year Plan review and the 15th Five-Year Plan outlook for beef cattle genetic improvement in China: Progress, problems, and countermeasures [J]. Chinese Livestock and Poultry Breeding, 2026, 22(10): 28-37.
[13]	Ping Yan, Qingbo Zheng, Ning Li, Siyiti Keremu, Li Fu. Exploration of genetic improvement progress and industrialization pathways for yaks [J]. Chinese Livestock and Poultry Breeding, 2026, 22(10): 10-14.