Abstract: In this experiment, 2kg Epimedium was used as raw material, and Epimedium polysaccharide was extracted by water extraction and alcohol precipitation method, sevage method and anion exchange column. The semen of 10 Simmental cattle was weighed and measured. Each semen was divided into 5 equal parts by a pipette. The Epimedium polysaccharide solution was diluted with ultrapure water to dilute the finished bovine frozen semen diluent. The polysaccharide concentrations were 8, 4, and 2 mg / mL, respectively. The control group did not add Epimedium polysaccharide, and the frozen semen vitality and deformity rate were detected after encapsulation and freezing. Bovine frozen semen containing Epimedium polysaccharide diluent was prepared for artificial insemination, and the difference of conception rate between polysaccharide frozen semen and ordinary frozen semen was compared. The results showed that 255.9134 g of crude polysaccharide with a sugar content of 58.86 % was obtained by water extraction and alcohol precipitation, and 51.2411 g of polysaccharide with a sugar content of 94.8 % was obtained by sevage method and anion exchange column after removing protein. When the concentration of Epimedium polysaccharide was 2 mg/mL, the thawing activity of frozen semen was (51 ± 3.7) %, the deformity rate was (21.15 ± 9.34) %, and the conception rate was 80.00 %, which was better than other groups. Conclusion : When the concentration of 94.8 % Epimedium polysaccharide is 2 mg / mL, the reproductive function of bovine sperm can be preserved to the maximum extent, and the conception rate of artificial insemination is the highest.

Key words: Epimedium; Separation and purification; Frozen semen; Artificial insemination