Abstract: In order to improve the breeding conception rate of local buffalo, the best insemination site for local buffalo deep insemination was studied and discussed. Buffaloes with natural estrus were divided into 4 groups, with 37 estrous cows in each group. Insemination was performed at the internal opening of the cervix, uterine body bifurcation, the greater curvature of the uterine horn on the follicular development side, and the lesser curvature of the uterine horn on the follicular development side. The mating and conception of buffaloes in each group were observed. Fifty experimental pregnant cows were randomly selected to observe the estrus, uterine infection, and mating and conception of cows within 2 months after weaning. The results showed that the conception rates of cervical internal opening, uterine body bifurcation, uterine horn greater curvature on the follicular development side, and uterine horn lesser curvature on the follicular development side were 54.05%, 45.94%, 64.87%, and 75.67%, respectively. The average conception rate of uterine horn lesser curvature was significantly higher than that of the other three groups (P<0.01). The estrus rate and conception rate of calves at 60 days after weaning were 72% and 75%, respectively. There were 46 cows. with white blood cells <10 by microscopic examination of mucus smear, accounting for 92%; blood cell analysis of 47 healthy cows, accounting for 94%. It can be seen that the best part of buffalo deep insemination is between the large and small curvatures of the uterine horn on the follicular development side, which can significantly improve the conception rate of local buffalo breeding.

Key words: buffalo, deep insemination, application, effect observation