不同同期发情处理及蛋白营养补给对非发情季节放牧生产欧拉羊发情率及血液激素水平影响

doi:10.19543/j.cnki.1673-4556.20260629.003

Abstract

Abstract:

Objective Oula sheep are an important meat-purpose breed of Tibetan sheep on the Qinghai–Tibet Plateau. However, due to pasture shortages during the long cold season and their seasonal estrus pattern, the estrus rate of grazing Oula sheep during the non-estrus season is extremely low, which severely limits batch production and farming efficiency. This study therefore sought to identify a cost-effective and easy-to-apply estrus synchronization protocol for grazing Oula sheep at the end of the cold season that would provide technical support for batch reproduction. Methods The study was conducted at a hornless Oula sheep cooperative in Henan Mongolian Autonomous County, Huangnan Prefecture, Qinghai Province. A total of 160 healthy, multiparous, 2.5-year-old hornless Oula ewes with similar body weights were randomly assigned to 16 groups of 10 animals each. Four nutritional levels were compared: natural grazing alone, and natural grazing supplemented with concentrates containing 16%, 18% or 20% crude protein. Three estrus synchronization protocols were also applied: two injections of cloprostenol (PG); insertion of a flurogestone acetate sponge (Progesterone-releasing intravaginal device, PRID) combined with a PG injection; and an injection of gonadorelin (Gonadotropin-releasing hormone, GnRH) combined with a PG injection. The experiment lasted 10 days. Blood samples were collected at the beginning and end of the experiment, and serum concentrations of estradiol (E2), progesterone (P), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured using enzyme-linked immunosorbent assay. Estrus rates were determined at the end of the experiment by teasing with a ram. Results Under natural grazing conditions during the non-estrus season, Oula sheep did not show estrus (0% estrus rate). After concentrate supplementation, natural estrus began to appear. Supplementation with 18% or higher protein concentrate increased the natural estrus rate to 30%. Treatment with exogenous hormones alone under natural grazing conditions produced estrus rates of 30%–40%. Combining nutritional supplementation with estrus synchronization treatment further increased estrus rates, with the highest rate (60%) observed in the group that received 18% protein supplementation combined with the GnRH+PG protocol. Hormone measurements revealed no significant differences in serum E2 or LH concentrations among the groups (P > 0.05). The GnRH+PG group had significantly higher serum FSH concentrations than the other groups (P < 0.05), while the PRID+PG group had significantly higher serum P concentrations than the other groups (P < 0.05). Conclusion At the end of the cold season on the Qinghai–Tibet Plateau, short-term supplementation with 18% or higher protein concentrate effectively induces natural estrus in Oula sheep during the non-estrus season, but the resulting estrus rate remains modest. Adding exogenous hormone treatment to nutritional supplementation improves the estrus rate further. The combination of short-term 18% protein supplementation with the GnRH+PG protocol achieves approximately 60% estrus synchronization at a manageable cost and is recommended for batch reproduction of grazing Oula sheep during the non-estrus season.

Key words: Synchronization of estrus, Grazing production, Non-estrus season, Tibetan sheep, Protein nutrition, Estrus rate

CLC Number:

S826

Yifan Li, Mingyi Yan, Sen Wu, Yongqin Li, Chengyun Li, Cairang Duojie, Yanqing Tang, Yanhua Tang. Effects of different estrus treatments and protein nutritional supplementation on estrus rate and blood hormone levels of Oula sheep in grazing during the non-estrus season[J].Chinese Livestock and Poultry Breeding, 2026, 22(7): 58-68.

Figures/Tables 6

Table 1

Table 2

Table 3

Table 4

Table 5

Table 6

References 42

[1]	周亚, 阎明毅, 杨桂梅, 等. 秋季补饲对不同年龄阶段欧拉羊血清生化指标的影响[J]. 饲料研究, 2024, 47(3): 1-5.
	ZHOU Y, YAN M Y, YANG G M, et al. Effects of autumn supplementary feeding on serum biochemical indexes of Oula sheep at different ages[J]. Feed Research, 2024, 47(3): 1-5.
[2]	刘梦, 阎明毅, 余忠祥, 等. 补饲对无角欧拉羊屠宰性能的影响[J]. 中国草食动物科学, 2022(5): 75-79.
	LIU M, YAN M Y, YU Z X, et al. Effects of supplementary feeding on slaughter performance of hornless oula sheep[J]. China Herbivore Science, 2022(5): 75-79.
[3]	李一凡, 阎明毅, 褚毅, 等. 青海省藏羊产业现状分析与发展建议[J]. 青海畜牧兽医杂志, 2026, 56(2): 32-38.
	LI Y F, YAN M Y, CHU Y, et al. Analysis of current situation and development suggestions of Tibetan sheep industry in Qinghai Province[J]. Chinese Qighai Journal of Animal and Veterinary Sciences, 2026, 56(2): 32-38.
[4]	吴森, 余忠祥, 阎明毅, 等. 青海地区欧拉羊本品种选育效果分析[J]. 黑龙江畜牧兽医, 2022(19): 45-53.
	WU S, YU Z X, YAN M Y, et al. Analysis on selective breeding effect of Oula sheep breed in Qinghai area[J]. Heilongjiang Animal Science and Veterinary Medicine, 2022(19): 45-53.
[5]	LI Y M, WANG Y H, YAN M Y, et al. Screening and functional transformation analysis of genes related to skeletal muscle development in supplemental-fed oula sheep[J]. Animals, 2025, 15(20): 3040.
[6]	朱士恩. 家畜繁殖学[M]. 6版. 北京: 中国农业出版社, 2015.
	ZHU S E. Animal Reproduction[M]. 6th ed. Beijing: China Agriculture Press, 2015.
[7]	巴合提努尔·哈德尔. 饲养方式及营养状况对受体羊同期发情、受胎率的影响[J]. 畜牧兽医科学（电子版）, 2021(19): 9-10.
	BAHETINUER H D E. Effects of feeding methods and nutritional status on estrus synchronization and conception rate of recipient sheep[J]. Graziery Veterinary Sciences (Electronic Version), 2021(19): 9-10.
[8]	田占伟. 利用营养水平诱导新疆哈萨克绵羊产后发情激素变化规律及部分相关基因表达研究[D]. 石河子: 石河子大学, 2015.
	TIAN Z W. The research of some related genes expression and the hormones changing rules of postpartum oestrus sheep after induced with different nutrition levels[D]. Shihezi: Shihezi University, 2015.
[9]	李月彩, 段春辉, 周玲莉, 等. 日粮能量、蛋白水平对燕山绒山羊育成母羊生长及生殖的影响[J]. 家畜生态学报, 2020, 41(8): 26-32.
	LI Y C, DUAN C H, ZHOU L L, et al. Effects of dietary energy and protein levels on growth performance and reproduction of Yanshan female Cashmere goats[J]. Acta Ecologae Animalis Domastici, 2020, 41(8): 26-32.
[10]	包时银, 田占伟, 刘鸽, 等. 非繁殖季节补饲精料诱导哈萨克羊发情及相关生殖激素变化规律的探究[J]. 黑龙江畜牧兽医, 2015(2): 9-13.
	BAO S Y, TIAN Z W, LIU G, et al. Exploration for the estrus induction in the Kazakh sheep using supplementary feeding with concentrate in the non-reproductive seasons and the variation regularity of the relative reproductive homones[J]. Heilongjiang Animal Science and Veterinary Medicine, 2015(2): 9-13.
[11]	应诗家. 黄体期不同饲喂量对湖羊卵泡发育、血液理化指标和卵泡内微环境的影响[D]. 南京: 南京农业大学, 2012.
	YING S J. Effects of different feeding levels during luteal phase on follicular development, blood biochemical parameters and intrafollicular microenvironment in Hu sheep[D]. Nanjing: Nanjing Agricultural University, 2012.
[12]	杨启林, 李善春, 李凯, 等. 不同饲喂方式对同期牦牛的发情率、血液生化指标和生殖激素的影响[J]. 中国饲料, 2024(18): 13-16.
	YANG Q L, LI S C, LI K, et al. Effects of different feeding methods on estrus rate, blood biochemical indexes and reproductive hormones of yaks in the same period[J]. China Feed, 2024(18): 13-16.
[13]	杨巧莓. 补饲+放牧和放牧两种饲养方式对牦牛同期发情发情率及代谢组学研究[D]. 西宁: 青海大学, 2024.
	YANG Q M. Effects of supplementary feeding plus grazing vs. grazing alone on estrus rate and metabolomics in yaks during synchronized estrus[D]. Xining: Qinghai University, 2024.
[14]	王晶晶, 刘昱成, 张振良, 等. 羊同期发情定时输精技术研究进展[J]. 中国畜禽种业, 2025, 21(4): 75-83.
	WANG J J, LIU Y C, ZHANG Z L, et al. Recent advances in the simultaneous Oestrus following timed insemination in ewes[J]. The Chinese Livestock and Poultry Breeding, 2025, 21(4): 75-83.
[15]	符汉宇, 苏奎源, 陈哲, 等. 西藏色瓦绵羊同期发情及人工授精技术应用研究[J]. 黑龙江畜牧兽医, 2025(4): 61-66.
	FU H Y, SU K Y, CHEN Z, et al. Study on the application of simultaneous estrus and artificial insemination technology in Xizang Sewa sheep[J]. Heilongjiang Animal Science and Veterinary Medicine, 2025(4): 61-66.
[16]	郑殿重, 刘玉峰, 李满雨, 等. 肉羊高效繁殖技术在生产实践中的应用[J]. 黑龙江动物繁殖, 2024(6): 33-36, 54.
	ZHENG D Z, LIU Y F, LI M Y, et al. Application of efficient breeding technology in sheep production[J]. Heilongjiang Journal of Animal Reproduction, 2024(6): 33-36, 54.
[17]	王安江, 张健, 李强, 等. 孕激素海绵栓+PG+PMSG处理与自然发情的山羊排卵率的比较[J]. 草食家畜, 1999(2): 18-19.
	WANG A J, ZHANG J, LI Q, et al. Comparison of ovulation rate between progesterone sponge suppository +PG+PMSG treatment and natural estrus goats[J]. Grass-Feeding Livestock, 1999(2): 18-19.
[18]	杨梅, 翁凡, 汪立芹, 等. 利用CIDR+PMSG对乏情期绵羊同期发情处理的研究[J]. 草食家畜, 2004(3): 38-39.
	YANG M, WENG F, WANG L Q, et al. Study on estrus synchronization of sheep with CIDR + PMSG during non - breeding seasons[J]. Grass-Feeding Livestock, 2004(3): 38-39.
[19]	朱栋栋, 孟宪煜, 韩高慧, 等. 不同同期发情方法对小尾寒羊发情效果的影响[J]. 畜牧与兽医, 2025, 57(4): 20-25.
	ZHU D D, MENG X Y, HAN G H, et al. Influence of different simultaneous estrus methods on the effect of estrus in Small-tailed Han sheep[J]. Animal Husbandry & Veterinary Medicine, 2025, 57(4): 20-25.
[20]	张文魁, 靳永平, 党梦皓. 利用GnRH+PMSG+PG对乏情期高原型藏羊进行同期发情处理[J]. 青海畜牧兽医杂志, 2025, 55(5): 60-61.
	ZHANG W K, JIN Y P, DANG M H. GnRH+PMSG+PG was used to treat plateau Tibetan sheep in estrus synchronization[J]. Chinese Qighai Journal of Animal and Veterinary Sciences, 2025, 55(5): 60-61.
[21]	杨巧莓, 舒适, 彭巍, 等. 不同孕酮含量硅胶阴道栓对牦牛生殖激素及排卵率的影响[J]. 中国畜牧杂志, 2024, 60(10): 224-227.
	YANG Q M, SHU S, PENG W, et al. Effects of silica gel vaginal suppository with different progesterone content on reproductive hormone and ovulation rate of yak[J]. Chinese Journal of Animal Science, 2024, 60(10): 224-227.
[22]	蒋如明, 韦英明, 凌泽继. 氯前列烯醇、孕酮阴道栓单独或结合其它激素处理本地母水牛后的发情和受胎情况[J]. 中国畜牧杂志, 2003, 39(6): 17-18.
	JIANG R M, WEI Y M, LING Z J. The estrous and conception rates in swamp buffaloes after synchronization by PGc, CIDR alone or cimhined with other hormones[J]. Chinese Journal of Animal Science, 2003, 39(6): 17-18.
[23]	马保华, 张红, 李键, 等. 山羊胚胎移植受体同期发情优化程序研究[J]. 西南农业学报, 2004, 17(5): 664-667.
	MA B H, ZHANG H, LI J, et al. Studies on optimized method of synchronized estrus in goats[J]. Southwest China Journal of Agricultural Sciences, 2004, 17(5): 664-667.
[24]	李秋艳, 张忠诚, 阎风祥, 等. 影响小尾寒羊同期发情效果因素的研究[J]. 畜牧兽医杂志, 2001, 20(2): 7-8, 11.
	LI Q Y, ZHANG Z C, YAN F X, et al. Stud on the influence factors of results atfecting small tailed Han sheeps induced heat[J]. Journal of Animal Science and Veterinary Medicine, 2001, 20(2): 7-8, 11.
[25]	高志敏, 雷安民, 窦忠英, 等. 波尔山羊胚胎移植技术的研究与应用[J]. 农业生物技术学报, 2001, 9(2): 167-170.
	GAO Z M, LEI A M, DOU Z Y, et al. Application and research of embryo transfer in Boer goats[J]. Journal of Agricultural Biotechnology, 2001, 9(2): 167-170.
[26]	张一玲, 渊锡藩, 尹海科, 等. 山羊同期发情技术的应用[J]. 畜牧兽医杂志, 2000, 19(5): 21-24.
	ZHANG Y L, YUAN X F, YIN H K, et al. Application of estrus synchronization technique in goat[J]. Journal of Animal Sciaence and Veterinary Medicine, 2000, 19(5): 21-24.
[27]	何冰梅, 金美多吉, 王品珍, 等. 西藏多玛绵羊同期发情处理及外周血液激素含量变化研究[J]. 中国畜牧杂志, 2023, 59(7): 159-163.
	HE B M, JIN M D J, WANG P Z, et al. Study on estrus synchronization and changes of hormone content in peripheral blood of dorma sheep in Xizang[J]. Chinese Journal of Animal Science, 2023, 59(7): 159-163.
[28]	贺小云, 狄冉, 胡文萍, 等. 绵羊季节性繁殖的神经内分泌研究进展[J]. 畜牧兽医学报, 2018, 49(1): 18-25
	HE X Y, DI R, HU W P, et al. Research progress on neuroendocrine of seasonal reproduction in sheep[J]. Chinese Journal of Animal and Veterinary Sciences, 2018, 49(1): 18-25.
[29]	任子珍, 贺小云, 储明星. miR-370-3p通过靶向EYA3基因参与绵羊光周期响应的调控机制[J]. 中国畜牧杂志, 2026, 62(4): 118-123.
	REN Z Z, HE X Y, CHU M X. miR-370-3p participates in the regulation mechanism of sheep photoperiod response by targeting EYA3 gene[J]. Chinese Journal of Animal Science, 2026, 62(4): 118-123.
[30]	NAKANE Y, YOSHIMURA T. Photoperiodic regulation of reproduction in vertebrates[J]. Annual Review of Animal Biosciences, 2019, 7: 173-194.
[31]	张玉珍, 马桂琳, 李红梅, 等. 甘南藏羊高效繁育及健康养殖技术[J]. 甘肃畜牧兽医, 2025, 55(5): 89-93.
	ZHANG Y Z, MA G L, LI H M, et al. Efficient breeding and healthy breeding technology of Gannan Tibetan sheep[J]. Gansu Animal and Veterinary Sciences, 2025, 55(5): 89-93.
[32]	李月彩. 日粮能量和蛋白质水平对燕山绒山羊育成羊生长及繁殖性能影响[D]. 保定: 河北农业大学, 2019.
	LI Y C. Effects of dietary energy and protein levels on growth and reproductive performance of developing Yanshan cashmere goats[D]. Baoding: Hebei Agricultural University, 2019.
[33]	艾克拜尔·艾合麦提, 程志跃, 阿布力皮孜·买买提, 等. 不同处理方法对湖羊初产母羊同期发情和繁殖性能的影响[J]. 畜牧与兽医, 2024, 56(11): 28-32.
	AIKEBAIER A H M T, CHENG Z Y, ABULIPIZI M M T, et al. Effects of different protocols of estrous synchronization on reproductive performance of primiparous ewes of Hu sheep[J]. Animal Husbandry & Veterinary Medicine, 2024, 56(11): 28-32.
[34]	谢周勋. 不同处理方法对戴云山羊同期发情效果的影响[J]. 畜牧兽医杂志, 2021, 40(6): 40-42.
	XIE Z X. Effects of different treatments on estrus synchronization in Daiyun goats[J]. Journal of Animal Science and Veterinary Medicine, 2021, 40(6): 40-42.
[35]	吴贤锋, 刘远, 张晓佩, 等. 不同处理方法对福清山羊同期发情效果的影响[J]. 中国草食动物科学, 2017, 37(6): 77-78.
	WU X F, LIU Y, ZHANG X P, et al. Effect of different treatment methods on estrus synchronization of Fuqing goats[J]. China Herbivore Science, 2017, 37(6): 77-78.
[36]	尚倩倩, 杜海东, 王跟辉, 等. 非繁殖季节不同处理方法诱导中卫山羊同期发情效果研究[J]. 中国畜牧杂志, 2025, 61(2): 192-195, 202.
	SHANG Q Q, DU H D, WANG G H, et al. Study on the effect of different treatments on estrus synchronization in Zhongwei goats during non-breeding season[J]. Chinese Journal of Animal Science, 2025, 61(2): 192-195, 202.
[37]	ROBERTS J L, MANI S K, WOLLER M J, et al. LHRH-(1–5): a bioactive peptide regulating reproduction[J]. Trends in Endocrinology & Metabolism, 2007, 18(10): 386-392.
[38]	EVANS G, BROOKS J, STRUTHERS W, et al. Superovulation and embryo recovery in ewes treated with gonadotrophin-releasing hormone agonist and purified follicle-stimulating hormone[J]. Reproduction, Fertility, and Development, 1994, 6(2): 247-252.
[39]	SHARIF B, HASSAN M, ARSHAD U, et al. Effect of eCG dose on ovarian haemodynamics, hormonal profiles and prolificacy rate when Oestrus was induced during low-breeding season in Beetal goats[J]. Reproduction in Domestic Animals, 2023, 58(1): 48-59.
[40]	MONNIAUX D, BARIL G, LAINE A L, et al. Anti-Mullerian hormone as a predictive endocrine marker for embryo production in the goat[J]. Reproduction, 2011, 142(6): 845-854.
[41]	华恒. 滩羊高效繁殖技术试验研究与生殖激素监测[D]. 杨凌: 西北农林科技大学, 2023.
	HUA H. Experimental study on efficient breeding technology and reproductive hormone monitoring of Tan sheep[D]. Yangling: Northwest A&F University, 2023.
[42]	高利华, 马国辅, 夏远方, 等. 小梅山猪性发育过程中生殖激素的变化规律[J]. 江苏农业科学, 2013, 41(9): 176-178.
	GAO L H, MA G F, XIA Y F, et al. Changes of reproductive hormones during sexual development of Xiaomeishan pigs[J]. Jiangsu Agricultural Sciences, 2013, 41(9): 176-178.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

序号NO.	未做同期发情药物处理 No estrus synchronization	同期发情处理1组 Estrus synchronization group 1	同期发情处理2组 Estrus synchronization group 2	同期发情处理3组 Estrus synchronization group 3
1	A组自然放牧	E组（A组+处理1）	I组（A组+处理2）	M组（A组+处理3）
2	B组（A组+16%蛋白精饲料）	F组（B组+处理1）	J组（B组+处理2）	N组（B组+处理3）
3	C组（A组+18%蛋白精饲料）	G组（C组+处理1）	K组（C组+处理2）	O组（C组+处理3）
4	D组（A组+20%蛋白精饲料）	H组（D组+处理1）	L组（D组+处理2）	P组（D组+处理3）

原料Ingredients/%	组别Groups
原料Ingredients/%	16%蛋白	18%蛋白	20%蛋白
玉米Corn	60.2	54.5	48.8
小麦Wheat	10	10	10
豆粕Soybean meal	16.1	21.8	27.5
菜籽粕Rapeseed meal	2	2	2
棉籽粕Cottonseed meal	2	2	2
玉米胚芽粕Corn germ meal	2	2	2
棕榈粕Palm kernel meal	2	2	2
食盐NaCl	1	1	1
石粉Limestone	1	1	1
小苏打NaHCO₃	0.1	0.1	0.1
预混料Premix¹	3.6	3.6	3.6
营养水平Nutritional level²
消化能DE/(MJ·kg^－1)	12.96	12.97	12.96
粗蛋白质CP	16.11	18.06	20.06
粗脂肪EE	3.79	4.13	4.44
粗纤维CF	4.67	4.43	4.24
中性洗涤纤维NDF	12.99	12.69	12.39
酸性洗涤纤维ADF	6.78	6.4	6.03
钙Ca	1.5	1.49	1.48
磷P	0.59	0.61	0.63

序号NO.	组别Groups	氯前列醇钠PG/(元·次^-1)	孕达海绵栓PRID/(元·次^-1)	戈那瑞林GnRH/(元·次^-1)	操作次数Number of operations/次	总计Total cost/(元·只^-1)
1	同期发情处理1组	2			2	4
2	同期发情处理2组	2	10		3	12
3	同期发情处理3组	2		3.5	3	9

序号NO.	组别Groups	处理Treatment	受测母羊数Number of ewes tested/只	发情母羊数Number of ewes in estrus/只	发情率Estrus rate/%
1	自然放牧	自然放牧	10	0	0
2	自然放牧+16%蛋白饲料	单独补饲	10	1	10
3	自然放牧+18%蛋白饲料	单独补饲	10	3	30
4	自然放牧+20%蛋白饲料	单独补饲	10	3	30
5	自然放牧	自然放牧+ GnRH+PG	10	4	40
6	自然放牧+16%蛋白饲料	补饲+ GnRH+PG	10	5	50
7	自然放牧+18%蛋白饲料	补饲+ GnRH+PG	10	6	60
8	自然放牧+20%蛋白饲料	补饲+ GnRH+PG	10	6	60

组别Groups	处理前Before treatment				发情羊（处理后）Estrus ewes (after treatment)
组别Groups	雌二醇E2/(pg·mL^-1)	促卵泡激素FSH/(mIU·mL^-1)	促黄体激素LH/(mIU·mL^-1)	孕酮P/(ng·mL^-1)	雌二醇E2/(pg·mL^-1)	促卵泡激素FSH/(mIU·mL^-1)	促黄体激素LH/(mIU·mL^-1)	孕酮P/(ng·mL^-1)
A	35.02±0.57	2.41±0.21	3.60±0.41	17.41±0.78	/	/	/	/
B	34.85±1.41	2.39±0.17	3.57±0.77	17.22±1.42	68.80	4.20	4.15	8.33
C	34.72±0.63	2.48±0.25	3.61±1.29	17.43±0.90	70.22±1.48	4.30±0.06^b	4.20±0.11	8.25±0.36^b
D	34.81±0.57	2.40±0.19	3.54±0.34	17.31±1.03	71.33±2.55	4.22±0.24^b	4.31±0.15	8.34±0.41^b
E	34.70±1.33	2.27±0.33	3.55±0.66	16.99±1.08	69.74±3.01	4.21±0.16^b	4.01±0.24	8.37±0.24^b
F	35.11±0.81	2.35±0.17	3.54±0.41	17.11±2.04	69.41±2.48	4.36±0.22^b	4.22±0.31	8.16±0.37^b
G	35.09±0.76	2.35±0.25	3.59±0.56	17.34±0.47	70.08±2.18	4.38±0.21^b	4.13±0.18	8.27±0.43^b
H	34.93±1.31	2.40±0.22	3.55±0.70	17.28±1.62	69.44±1.08	4.22±0.24^b	4.37±0.22	8.46±0.23^b
I	34.88±1.42	2.36±0.17	3.49±1.36	17.30±0.82	70.15±1.37	4.27±0.16^b	4.28±0.17	8.96±0.18^a
J	35.10±1.88	2.27±0.31	3.52±0.64	17.36±0.55	69.15±2.77	4.39±0.16^b	4.26±0.27	9.19±0.33^a
K	34.65±0.94	2.52±0.32	3.58±1.29	17.50±1.03	70.97±1.38	4.27±0.13^b	4.29±0.30	9.39±0.35^a
L	34.77±2.46	2.41±0.29	3.58±1.33	17.35±0.81	68.49±1.91	4.33±0.18^b	4.35±0.11	9.46±0.42^a
M	34.67±2.00	2.29±0.41	3.33±0.44	16.88±2.04	70.01±2.34	4.47±0.21^ab	4.31±0.21	8.14±0.17^b
N	35.01±1.84	2.37±0.22	3.34±0.47	17.03±1.73	72.22±1.27	4.91±0.24^a	4.31±0.18	8.27±0.39^b
O	34.77±1.31	2.41±0.37	3.46±0.36	17.24±1.09	73.41±2.38	4.86±0.18^a	4.59±0.18	8.31±0.46^b
P	35.23±0.97	2.27±0.41	3.70±0.14	16.88±2.07	73.83±3.09	4.79±0.19^a	4.59±0.20	8.24±0.38^b

Effects of different estrus treatments and protein nutritional supplementation on estrus rate and blood hormone levels of Oula sheep in grazing during the non-estrus season

RichHTML

PDF (PC)

Abstract

Cite this article

share this article

Figures/Tables 6

References 42

Related Articles 2

Metrics

Comments

Recommended 0

项目Item	组别Groups
项目Item	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P
受测母羊数Number of ewes tested/只	10	10	10	10	10	10	10	10	10	10	10	10	10	10	10	10
发情母羊数Number of ewes in estrus/只	0	1	3	3	3	4	5	5	4	4	5	6	4	5	6	6
发情率Estrus rate/%	0	10	30	30	30	40	50	50	40	40	50	60	40	50	60	60

[1]	Jinnan Li, Jinming Wang, Shizhi Wang, Lan Zhu, Dongmei Yang, Caijuan Liu, Kaimei Yang, Haolin Chen, Qingyong Shao. Analysis of population genetic structure of Yongsheng Red-bone sheep based on SNP chip [J]. Chinese Livestock and Poultry Breeding, 2026, 22(7): 38-44.
[2]	Yuxin Cai, Meijie Wang, Xingwang Liu, Jialiang Zhang, Fang Li, Haotong Yang, Yuxin Yang, Xiaoyi Zhang, Man Bai. Analysis of molecular characteristics of the CDS region of the sheep CYP17A1 gene and its expression in testes at different developmental stages [J]. Chinese Livestock and Poultry Breeding, 2026, 22(5): 43-51.