绵羊CYP17A1基因CDS区分子特征分析及其在不同时期睾丸中的表达研究

doi:10.19543/j.cnki.1673-4556.20260407.001

中国畜禽种业 ›› 2026, Vol. 22 ›› Issue (5): 43-51.doi: 10.19543/j.cnki.1673-4556.20260407.001cstr: 32418.14.j.cnki.1673-4556.20260407.001

所属专题：功能基因组学

绵羊CYP17A1基因CDS区分子特征分析及其在不同时期睾丸中的表达研究

蔡雨欣(), 王美桀, 刘兴旺, 张嘉良, 李放, 杨浩彤, 杨宇欣, 张笑怡, 白曼()

沈阳农业大学动物科学与医学学院，辽宁沈阳 110866

收稿日期:2025-10-10 出版日期:2026-03-26 发布日期:2026-06-17
通讯作者: 白曼 E-mail:18847908342@163.com;doctorbaiman@163.com
作者简介:
蔡雨欣（2000—），女，内蒙古锡林郭勒人，硕士研究生，研究方向：绵羊、山羊遗传育种与繁殖，E-mail：18847908342@163.com。
基金资助:
辽宁省教育厅基础研究项目(LSNQN202005); 辽宁省科技厅博士科研启动基金计划项目(2021-BS-137)

Analysis of molecular characteristics of the CDS region of the sheep CYP17A1 gene and its expression in testes at different developmental stages

Yuxin Cai(), Meijie Wang, Xingwang Liu, Jialiang Zhang, Fang Li, Haotong Yang, Yuxin Yang, Xiaoyi Zhang, Man Bai()

College of Animal Science and Medicine, Shenyang Agricultural University, ShenYang, 110866, Liaoning

Received:2025-10-10 Online:2026-03-26 Published:2026-06-17
Contact: Man Bai E-mail:18847908342@163.com;doctorbaiman@163.com

摘要/Abstract

摘要：

目的为探究CYP17A1在小尾寒羊睾丸发育中的动态表达特征，明确其功能和表达规律，为后续深入研究提供参考。方法该研究分别通过不同的生物信息学软件对绵羊CYP17A1的分子特征展开研究，并构建系统发育进化树；同时，运用实时荧光定量PCR（qRT-PCR）与蛋白免疫印迹（Western Blot）技术检测CYP17A1在不同月龄（2、6、12月龄）小尾寒羊睾丸中的表达情况。结果绵羊CYP17A1基因CDS区全长1576 bp，与山羊和牛等反刍动物亲缘关系最近，共编码509个氨基酸，CYP17A1蛋白分子式为C₂₆₀₅H₄₁₄₀N₆₉₆S₁₅，分子量为57.39 kDa，理论等电点8.60，是一种具有磷酸化位点但不存在信号肽的轻微亲水性蛋白。二级结构和三级结构均以α-螺旋、β-转角、延伸链和无规卷曲构成；CYP17A1基因和蛋白在3个时期睾丸组织中均有表达，且表达量均随着月龄增长呈逐级下降的趋势，即2月龄表达量最高，6月龄的表达量次之，12月龄的表达量最低。结论由此可见，CYP17A1基因影响小尾寒羊睾丸发育，并且对睾丸发育起到重要的调控作用。

关键词: 小尾寒羊, 睾丸组织, CYP17A1, 分子特征分析

Abstract:

Objective This study was conducted to investigate the dynamic expression characteristics of CYP17A1 during testicular development in Small-tail han sheep, clarify its biological function and expression pattern, and provide a theoretical reference for further research. Method In this study, multiple bioinformatics tools were used to analyze the molecular characteristics of sheep CYP17A1 and construct a phylogenetic tree. Meanwhile, quantitative real‑time polymerase chain reaction (qRT‑PCR) and Western blot were performed to detect the expression of CYP17A1 in the testes of Small‑Tail Han sheep at 2, 6, and 12 months of age. Result The full length of the CDS region of the sheep CYP17A1 gene is 1576 bp, and it shows the closest genetic relationship with ruminants such as goats and cattle. The gene encodes 509 amino acids. The molecular formula of the CYP17A1 protein is C₂₆₀₅H₄₁₄₀N₆₉₆S₁₅, with a molecular weight of 57.39 kDa and a theoretical isoelectric point of 8.60. It is a slightly hydrophilic protein that contains phosphorylation sites but has no signal peptide. The secondary and tertiary structures of CYP17A1 are mainly composed of α-helix, β-sheet, extended strand, and random coil. Both the CYP17A1 gene and its protein were expressed in testicular tissue at all three developmental stages, and their expression levels showed a gradually decreasing trend with increasing age: the highest expression was observed at 2 months of age, followed by 6 months of age, and the lowest at 12 months of age. Conclusion Taken together, these findings demonstrate that CYP17A1 is involved in and exerts an essential regulatory effect on testicular development in Small-tail han sheep.

Key words: Small-tailed han sheep, Testicular tissue, CYP17A1, Molecular characterization

中图分类号:

S826

蔡雨欣, 王美桀, 刘兴旺, 张嘉良, 李放, 杨浩彤, 杨宇欣, 张笑怡, 白曼. 绵羊CYP17A1基因CDS区分子特征分析及其在不同时期睾丸中的表达研究[J]. 中国畜禽种业, 2026, 22(5): 43-51.

Yuxin Cai, Meijie Wang, Xingwang Liu, Jialiang Zhang, Fang Li, Haotong Yang, Yuxin Yang, Xiaoyi Zhang, Man Bai. Analysis of molecular characteristics of the CDS region of the sheep CYP17A1 gene and its expression in testes at different developmental stages[J]. Chinese Livestock and Poultry Breeding, 2026, 22(5): 43-51.

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软件Software	网址Website URL	功能Function
Expasy	http://web.expasy.org/protparam/	理化性质分析
SignalP	https://services.healthtech.dtu.dk/services/SignalP-6.0/	信号肽预测
TMHMM	https://services.healthtech.dtu.dk/service.php TMHMM-2.0	跨膜结构域预测
NetPhos	https://services.healthtech.dtu.dk/services/NetPhos-3.1/	磷酸化位点预测
DictyOGlyc	https://services.healthtech.dtu.dk/services/DictyOGlyc-1.1/	O-（α）-GlcNAc糖基化位点预测
NetNGlyc	https://services.healthtech.dtu.dk/services/NetNGlyc-1.0/	N-糖基化位点预测
Prabi	https://npsa-prabi.ibcp.fr/cgi-bin/npsa_automat.pl page=/NPSA/npsa_server.html	二级结构预测
SWISS-MODEL	https://swissmodel.expasy.org/	三级结构预测
PSORT Ⅱ	https://psort.hgc.jp/form2.html	亚细胞定位

物种名 Species name	NCBI登录号 NCBI login number	相似性 Similarity/%
山羊Capra hircus	NM_001314145.1	99.15
牛Bos taurus	NM_174304.3	96.93
白尾鹿Odocolieus virginianus	XM_020897894.2	96.27
猪Sus scrofa	NM_214428.1	85.62
驴Equus asinus	XM_014852085.3	82.94
马Equus caballus	NM_001082523.1	82.75
猫Felis catus	NM_001009371.2	82.75
犬Canis lupus familiaris	XM_038440431.1	81.76
智人Homo sapiens	NM_000102.4	80.26
兔Oryctolagus cuniculus	XM_002718585.5	77.91
鼠Mus musculus	NM_007809.3	72.09

氨基酸Amino acid	数量Quantity	比例Proportion/%
丙氨酸 Ala（A）	32	6.3
精氨酸 Arg（R）	22	4.3
天冬酰胺 Asn（N）	25	4.9
天冬氨酸 Asp（D）	24	4.7
半胱氨酸 Cys（C）	5	1.0
谷氨酰胺 Gln（Q）	23	4.5
谷氨酸 Glu（E）	30	5.9
甘氨酸 Gly（G）	27	5.3
组氨酸 His（H）	15	2.9
异亮氨酸 Ile（I）	36	7.1
亮氨酸 Leu（L）	70	13.8
赖氨酸 Lys（K）	36	7.1
蛋氨酸 Met（M）	10	2.0
苯丙氨酸 Phe（F）	27	5.3
脯氨酸 Pro（P）	28	5.5
丝氨酸 Ser（S）	35	6.9
苏氨酸 Thr（T）	23	4.5
色氨酸 Trp（W）	7	1.4
酪氨酸 Tyr（Y）	7	1.4
缬氨酸 Val（V）	27	5.3

绵羊CYP17A1基因CDS区分子特征分析及其在不同时期睾丸中的表达研究

Analysis of molecular characteristics of the CDS region of the sheep CYP17A1 gene and its expression in testes at different developmental stages

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