中国畜禽种业 ›› 2022, Vol. 18 ›› Issue (1): 50-52.

• 试验研究 • 上一篇    下一篇

乙酰水杨酸对绵羊精子常温保存的影响

陈洁梅   

  1. 安徽农业大学 230031
  • 出版日期:2022-01-26 发布日期:2022-02-18
  • 作者简介:陈洁梅(1992-),女,汉族,甘肃省天水市人,硕士,研究方向:动物胚胎工程。

  • Online:2022-01-26 Published:2022-02-18

摘要: 为探究乙酰水杨酸对精子活力、质膜和顶体的保护作用。试验选取7只精子品质优良的绵羊作为采精对象。将精液稀释后分为空白对照组、A1组(20 mm/L)、A2组(50 mm/L)、A3组(100 mm/L),重复5次。用SCA软件检测精子活力、低渗肿胀试验检测精子的质膜完整性(Membrane integrity,MI);吉姆萨染色检测精子的顶体完整性(Acrosome integrity,AI)。结论50 mM的ASA有利于精子的常温保存。

关键词: 绵羊精子, 常温保存, 乙酰水杨酸, 质膜完整性, 顶体完整性

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