摘要: 为探究乙酰水杨酸对精子活力、质膜和顶体的保护作用。试验选取7只精子品质优良的绵羊作为采精对象。将精液稀释后分为空白对照组、A1组(20 mm/L)、A2组(50 mm/L)、A3组(100 mm/L),重复5次。用SCA软件检测精子活力、低渗肿胀试验检测精子的质膜完整性(Membrane integrity,MI);吉姆萨染色检测精子的顶体完整性(Acrosome integrity,AI)。结论50 mM的ASA有利于精子的常温保存。
[1] Nishio T.,Usami M.,Awaji M.,et al.Dual effects of acetylsalicylic acid on ERK signaling and Mitf transcription lead to inhibition of melanogenesis[J].Molecular and Cellular Biochemistry,2016,412(1-2):101-110. [2] Hockertz S., Schettler T., Rogalla K.Effect of acetylsalicylic acid, ascorbate and ibuprofen on the macrophage system[J].Arzneimittel-Forschung,1992,42(8):1062-1068. [3] Ruili Y.Acetylsalicylic acid treatment enhanced immunomodulatory function of mesenchymal stem cells derived from gingiva[J].Beijing da xue xue bao.Yi xue ban=Journal of Peking University. Health sciences,2017,49(5):872-877. [4] Michalska-Malecka K.,Regucka A.,Spiewak D.,et al.Does the use of acetylsalicylic acid have an influence on our vision[J].Clinical Interventions in Aging,2016,11:1567-1574. [5] Liu Y., Chen C., Liu S., et al.Acetylsalicylic acid treatment improves differentiation and immunomodulation of SHED[J].Journal of Dental Research,2015,94(1):209-218. [6] Lucio C.F., Brito M.M., Angrimani D., et al. Lipid composition of the canine sperm plasma membrane as markers of sperm motility[J].Reproduction in Domestic Animals,2017,52(2):208-213. [7] Panagodage S., Yong H.E., Da Silva Costa F., et al. Low-Dose Acetyl-salicylic Acid Treatment Modulates the Production of Cytokines and Improves Trophoblast Function in an in Vitro Model of Early-Onset Preeclampsia[J].The American Journal of Pathology,2016,186(12):3217-3224. [8] Emami N.H., Lafout F.M., Mohammadghasemi F. Administration of melatonin protects against acetylsalicylic acid-induced impairment of male reproductive function in mice[J].Iranian Journal of Basic Medical Science,2018,21(2):124-129. [9] Damasceno D.C., Volpato G.T., Person O.C., et al. Effect of acetylsalicylic acid on the reproductive performance and on offspring from wistar rats[J]. Revista da Associacao Medica Brasileira(1992),2002,48(4):312-316. [10] Vyas A., Ram H., Purohit A., et al.Adverse Effects of Subchronic Dose of Aspirin on Reproductive Profile of Male Rats[J].Journal of Pharmaceutics(Cairo),2016,(2016-4-12):6585430. |
No related articles found! |
|