摘要： 本试验拟克隆白来航鸡干扰素刺激基因12（ISG12）,并对其进行生物信息学预测分析。根据ncbi发表的原鸡ISG12基因序列（登录号：BN000223.1）,设计ISG12基因特异性扩增引物。提取白来航鸡的肝脏、脾脏RNA,并反转录为cDNA,利用RT-PCR技术扩增白来航鸡ISG12基因序列,并利用生物软件对其编码蛋白进行生物信息学分析预测。结果显示,白来航鸡ISG12基因CDS区为324bp,编码107个氨基酸。ISG12编码蛋白理论分子量为10.28 kD,理论等电点（PI）为10.18,不稳定系数为59.46,脂肪系数为41.12。存在3个明显的亲水区,整条多肽链表现为亲水性;无信号肽,不存在跨膜区;存在21个潜在的磷酸化位点,6个潜在的O-糖基化位点,不含N-糖基化修饰位点;含有3个抗原决定簇区域。本试验成功克隆了白来航鸡ISG12基因,为深入了解ISG12编码蛋白的功能及进一步阐述其抗病毒的机理提供了科学依据。
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