摘要： 为了研究黄曲霉毒素B1对脾脏和肝脏的影响,试验选取三周龄小鼠20只,按体重随机分成2组,每组10只。对照做健康饮食喂养,灌喂200μg/(kg·d)玉米油;黄曲霉毒素B1组每日灌胃用玉米油配制的黄曲霉毒素B1 200μg/(kg·d),试验连续28d。结果表明,黄曲霉毒素B1会对脾脏和肝脏造成损伤,扰乱正常生理功能,降低过氧化氢酶,三磷酸腺苷酶和还原型谷胱甘肽过氧化物酶的含量,增加丙二醛的含量,以此促进脂质氧化造成脾脏和肝脏的损伤。可见,黄曲霉毒素B1对脾脏和肝脏均有毒害作用。
| [1] Pickova D, Ostry V, Toman J, et al. Aflatoxins: history, significant milestones, recent data on their toxicity and ways to mitigation[J]. Toxins, 2021, 13(6):399. [2] Arino A, Herrera M, Estopanan G, et al. Aflatoxins in bulk and pre-packed pistachios sold in Spain and effect of roasting[J]. Food Control, 2009, 20(9):811-814. [3] Yazdanpanah H, Mohammadi T, Abouhossain G, et al. Effect of roasting on degradation of Aflatoxins in contaminated pistachio nuts[J]. Food & Chemical Toxicology, 2005, 43(7):1135-1139. [4] 李彦云. 饲料中黄曲霉毒素的危害及中毒的预防[J]. 现代畜牧科技, 2010(8):38-39. [5] Wills E D. Mechanism of lipid peroxide formation in animal tissues[J]. Biochem J, 1966(99):667-676. [6] Calabrese V, Lodi R, Tonon C, et al. Oxidative stress, mitochondrial dysfunction and cellular stress response in Friedreich's ataxia[J]. Journal of the Neurological Sciences, 2005, 233(1-2):145-162. [7] Eaton D L, Gallagher E P. Mechanisms of Aflatoxin Carcinogenesis[J]. Annual Review of Pharmacology and Toxicology, 1994, 34(1):135-172. [8] Guengerich F P, Johnson W W, Shimada T, et al. Activation and detoxication of aflatoxin B1[J]. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1998, 402(1-2):121-128. [9] Baertschi S W, Raney K D, Shimada T, et al. Comparison of rates of enzymic oxidation of aflatoxin B1, aflatoxin G1, and sterigmatocystin and activities of the epoxides in forming guanyl-N7 adducts and inducing different genetic responses[J]. Chemical Research in Toxicology, 1989, 2(2):114-122. [10] Johnson W W, Harris T M, Guengerich F P. Kinetics and Mechanism of Hydrolysis of Aflatoxin B1exo-8, 9-Epoxide and Rearrangement of the Dihydrodiol[J]. Journal of the American Chemical Society, 1996, 118(35):8213-8220. [11] Ray P D, Huang B, Tsuji Y. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling[J]. Cellular Signalling, 2012, 24(5):981-990. [12] Wei Y, Rector RS, Thyfault JP, et al. Nonalcoholic fatty liver disease and mitochondrial dysfunction[J]. World journal of gastroenterology, 2008, 14(2):193-199. [13] Auger C, Alhasawi A, Contavadoo M, et al. Dysfunctional mitochondrial bioenergetics and the pathogenesis of hepatic disorders[J]. Frontiers in Cell and Developmental Biology, 2015(3):40. [14] Sies H. Role of reactive oxygen species in biological processes[J]. Klinische Wochenschrift, 1991, 69(21-23):965-968. |
| [1] | 马浩海, 袁正宁, 刘静, 张西锋. 黄曲霉毒素B1对动物危害作用研究[J]. 中国畜禽种业, 2022, 18(11): 3-8. |
| [2] | . 双黄连对内毒素诱导小鼠肝脏组织结构的影响[J]. 中国畜禽种业, 2019, 15(1): 0-. |
|
||
